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KMID : 0359320000400020325
Korean Journal of Veterinary Research
2000 Volume.40 No. 2 p.325 ~ p.332
Studies on the characteristics of Lactobacillus plantarum isolated from oat silage



Abstract
The growth characteristics and the cellular protein patterns of the Lactobacillus plantarum isolated and identified from oat silage were examined in order to confirm whether it will be used practically as probiotics or not. L plantarum was identified by morphological and biochemical tests including of final conforming by API 50CHL kit. The cultivation in MRS broth of the strain under the condition of different temperature, proved that they grew into 2.0¡¿ 10^9 in 25¡É, into 1.4¡¿10^9 in 35¡É but they decreased into 4.5¡¿10^5 growth in 45¡É. The comparison of the growth by measurement of O.D600§¬ value after 24 hour cultivation between L plantarum and commercial probiotics, showed that the strain had a higher growth than commercial as 1.841 : 1.623. The measurement of it under bile acid¢¥s existence, indicated that this isolation was not influenced by bile acid and the tolerance was 3.2¡¿10^9, 3.9¡¿10^9 and 3.2¡¿10^9, respectively, when each of 0%, 1%, and 2% oxigall existed. The examination of their antibiotics susceptibility by disk diffusion test, proved that L plantarum showed resistance against danofloxacin(5mcg), gentamycin(10mcg), kanamycin(30mcg), neomycin(30mcg) and streptomycin(10mcg). Based upon the test of the bacteriocin formation of this L plantarum, it was found out that the inhibition zone was not formed. In growth of L plantarum and E coli in nutrient broth, all E coli died out within 6 hours after cultures.
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